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KMID : 1161519990030040413
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Animal Cells and Systems
1999 Volume.3 No. 4 p.413 ~ p.415
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Identification of a regulatory element required for 3'-end formation in transcripts of rhp51+, a recA homolog of the fission yeast Schizosaccharomyces pombe
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Jang Yeun-Kyu
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Abstract
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Our previous report demonstrated that the rhp51 + , a recA and RAD51 homolog of the fission yeast, encodes three transcripts of 1.9, 1.S and 1.3 kb which have at least six polyadenylation sites. The 3'?end of the gene alone can direct the formation of multiple, discrete 3’ ends of the transcripts. To identify the regulatory element required for the 3'?end formation of rhp51+ , deletion mapping analysis was performed. Northern blot analysis revealed that the 254?bp DIM A fragment including 4 distinct poly (A) sites downstream from the Hindlll site, is crucial for normal 3'?end formation. Deletion of the 3?terminal AU rich region caused appearance of read?through RNA, leading to enhancement of survival rate of the rhp51 deletion mutant in response to DNA damaging agent, methyl methane sulfonate (MMS). The results imply that the rhp51 system may be useful for molecular analysis of the 3'?end formation of RNA in the fission yeast.
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KEYWORD
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Fission yeast, rhp51 +, 3'-End formation, Polyadenylation site
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